NSJ Bioreagents

p57 Antibody / p57Kip2

Product Code:
 
NSJ-RQ7967
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the p57Kip2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
IHC staining of FFPE human lung cancer tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human cervical cancer tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human esophageal squamous carcinoma tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human rat kidney tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
IHC staining of FFPE human mouse kidney tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 6
Western blot testing of 1) human Jurkat, 2) human HepG2, 3) human A549, 4) human ThP-1, 5) human Raji, 6) human SW620, 7) human Caco-2, 8) human HEK293, 9) rat PC-12 and 10) mouse NIH 3T3 cell lysate with p57Kip2 antibody. Predicted molecular weight ~32 kDa but can be observed at ~57 kDa.
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IHC staining of FFPE human lung cancer tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rat kidney tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human mouse kidney tissue with p57Kip2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human HepG2, 3) human A549, 4) human ThP-1, 5) human Raji, 6) human SW620, 7) human Caco-2, 8) human HEK293, 9) rat PC-12 and 10) mouse NIH 3T3 cell lysate with p57Kip2 antibody. Predicted molecular weight ~32 kDa but can be observed at ~57 kDa.

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CodeSizePrice
NSJ-RQ7967-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Description:
Cyclin-dependent kinase inhibitor 1C (p57, Kip2), also known as CDKN1C, is a protein which in humans is encoded by the CDKN1C imprinted gene. It is mapped to 11p15.4. This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amio acids ARLAELNAEDQNRWD were used as the immunogen for the p57Kip2 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P49918