NSJ Bioreagents

FAF1 Antibody / FAS-ass°Ciated factor 1

Product Code:
 
NSJ-RQ7977
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the FAS-ass°Ciated factor 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with FAS-associated factor 1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human 293T, 3) human K562, 4) human ThP-1, 5) rat brain, 6) rat testis, 7) mouse brain and 8) mouse testis tissue lysate with FAS-associated factor 1 antibody. Expected molecular weight ~74 kDa.
3 / 3
Flow cytometry testing of human K562 cells with FAS-associated factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FAS-associated factor 1 antibody.
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Immunofluorescent staining of FFPE human A549 cells with FAS-associated factor 1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human K562, 4) human ThP-1, 5) rat brain, 6) rat testis, 7) mouse brain and 8) mouse testis tissue lysate with FAS-associated factor 1 antibody. Expected molecular weight ~74 kDa.
Flow cytometry testing of human K562 cells with FAS-associated factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FAS-associated factor 1 antibody.

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CodeSizePrice
NSJ-RQ7977-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Fas-associated factor-1 (FAF1) is a Fas-binding pro-apoptotic protein that is a component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 is involved in negative regulation of NF-kappaB activation. In addition to the previously known function as a component of the Fas death-inducing signaling complex, i.e. NF-kappaB activity suppressor by cytoplasmic retention of NF-kappaB p65 via physical interaction. hFAF1 was expressed abundantly in testis, skeletal muscle, and heart as 2.8 kb mRNA. Polyclonal antibody against hFAF1 detected 74 kD protein, a deduced protein size from the ORF and 40 kD protein in some cell lines.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R91-E650) was used as the immunogen for the FAS-associated factor 1 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9UNN5