NSJ Bioreagents

KHSRP Antibody / FUBP2

Product Code:
 
NSJ-RQ7979
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the FUBP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 10
Western blot testing of 1) rat brain, 2) rat stomach, 3) mouse brain and 4) mouse stomach tissue lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
2 / 10
Western blot testing of human 1) HEK293, 2) HeLa, 3) MCF7, 4) Jurkat, 5) K562, 6) Caco-2 and 7) U-937 cell lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
3 / 10
Immunofluorescent staining of FFPE human Caco-2 cells with FUBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 10
IHC staining of FFPE human lung cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human gallbladder adenocarcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human liver cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE human mouse brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
IHC staining of FFPE human rat brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 10
Flow cytometry testing of human K562 cells with FUBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FUBP2 antibody.
❮❯

Western blot testing of 1) rat brain, 2) rat stomach, 3) mouse brain and 4) mouse stomach tissue lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
Western blot testing of human 1) HEK293, 2) HeLa, 3) MCF7, 4) Jurkat, 5) K562, 6) Caco-2 and 7) U-937 cell lysate with FUBP2 antibody. Predicted molecular weight ~73 kDa.
Immunofluorescent staining of FFPE human Caco-2 cells with FUBP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gallbladder adenocarcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human mouse brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rat brain tissue with FUBP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human K562 cells with FUBP2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FUBP2 antibody.

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CodeSizePrice
NSJ-RQ7979-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Far upstream element-binding protein 2 is a protein that in humans is encoded by the KHSRP gene. The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D116-R478) was used as the immunogen for the FUBP2 antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q92945