NSJ Bioreagents

NDUFAF4 Antibody / HRPAP20 / C6orf66

Product Code:
 
NSJ-RQ8084
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the HRPAP20 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
Immunofluorescent staining of FFPE human intestinal cancer tissue with HRPAP20 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 8
Immunofluorescent staining of FFPE human HeLa cells with HRPAP20 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 8
IHC staining of FFPE human lung adenocarcinoma tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human ovarian tumor tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human rectum adenocarcinoma tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE human liver cancer tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of human 1) 293T, 2) MDA-MB-453, 3) K562 and 4) Ramos cell lysate with HRPAP20 antibody. Predicted molecular weight ~20 kDa.
8 / 8
Flow cytometry testing of fixed and permeabilized human U937 cells with HRPAP20 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HRPAP20 antibody.
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Immunofluorescent staining of FFPE human intestinal cancer tissue with HRPAP20 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human HeLa cells with HRPAP20 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung adenocarcinoma tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian tumor tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectum adenocarcinoma tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with HRPAP20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) 293T, 2) MDA-MB-453, 3) K562 and 4) Ramos cell lysate with HRPAP20 antibody. Predicted molecular weight ~20 kDa.
Flow cytometry testing of fixed and permeabilized human U937 cells with HRPAP20 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HRPAP20 antibody.

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CodeSizePrice
NSJ-RQ8084-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
NADH:ubiquinone oxidoreductase complex assembly factor 4 (NDUFAF4), also known as Hormone-regulated proliferation-associated protein of 20 kDa (HRPAP20) and C6orf66, is a protein that in humans is encoded by the NDUFAF4 gene. NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K24-K175) was used as the immunogen for the HRPAP20 antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
Q9P032