NSJ Bioreagents

PRP19 Antibody / NMP200 / SNEV

Product Code:
 
NSJ-RQ8091
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SNEV antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with SNEV antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HeLa, 2) Jurkat, 3) A431 and 4) MCF7 cell lysate with SNEV antibody. Predicted molecular weight ~55 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human JK cells with SNEV antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SNEV antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with SNEV antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) A431 and 4) MCF7 cell lysate with SNEV antibody. Predicted molecular weight ~55 kDa.
Flow cytometry testing of fixed and permeabilized human JK cells with SNEV antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SNEV antibody.

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CodeSizePrice
NSJ-RQ8091-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Pre-mRNA-processing factor 19, also called PRP19, SNEV (Senescence evasion factor) and NMP200 (Nuclear matrix protein 200), is a protein that in humans is encoded by the PRPF19 gene. Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm; nuclear speck; and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q51-H359) was used as the immunogen for the SNEV antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9UMS4