NSJ Bioreagents

EIF5 Antibody / Eukaryotic translation initiation factor 5

Product Code:
 
NSJ-RQ8103
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Eukaryotic translation initiation factor 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 12
IHC staining of FFPE human esophageal squamous carcinoma tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 12
IHC staining of FFPE human placental tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 12
IHC staining of FFPE human renal cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 12
IHC staining of FFPE human lung cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE human lung cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE human gastric cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE human gastric cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE mouse brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 12
IHC staining of FFPE mouse brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 12
IHC staining of FFPE rat brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 12
Western blot testing of human 1) K562, 2) HeLa, 3) HepG2, 4) PC-3 and 5) Caco-2 cell lysate with Eukaryotic translation initiation factor 5 antibody. Predicted molecular weight ~49 kDa.
12 / 12
Flow cytometry testing of human K562 cells with Eukaryotic translation initiation factor 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eukaryotic translation initiation factor 5 antibody.
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IHC staining of FFPE human esophageal squamous carcinoma tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) HeLa, 3) HepG2, 4) PC-3 and 5) Caco-2 cell lysate with Eukaryotic translation initiation factor 5 antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of human K562 cells with Eukaryotic translation initiation factor 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eukaryotic translation initiation factor 5 antibody.

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CodeSizePrice
NSJ-RQ8103-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Eukaryotic translation initiation factor 5 is a protein that in humans is encoded by the EIF5 gene. Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D11-D423) was used as the immunogen for the Eukaryotic translation initiation factor 5 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P55010