NSJ Bioreagents

PYGL Antibody / Glycogen phosphorylase, Liver

Product Code:
 
NSJ-RQ8159
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PYGL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human 293T cells with PYGL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PYGL antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human 293T, 4) rat liver and 5) mouse liver tissue lysate with PYGL antibody. Predicted molecular weight ~97 kDa.
❮❯

Flow cytometry testing of fixed and permeabilized human 293T cells with PYGL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PYGL antibody.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human 293T, 4) rat liver and 5) mouse liver tissue lysate with PYGL antibody. Predicted molecular weight ~97 kDa.

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CodeSizePrice
NSJ-RQ8159-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Glycogen phosphorylase, liver form (PYGL), also known as human liver glycogen phosphorylase (HLGP), is an enzyme that in humans is encoded by the PYGL gene on chromosome 14. This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K313-K804) was used as the immunogen for the PYGL antibody.
Purity:
Antigen affinity purified
Uniprot #:
P06737