NSJ Bioreagents

SESN1 Antibody / Sestrin 1 / SEST1 / PA26

Product Code:
 
NSJ-RQ8209
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PA26 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with PA26 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human 293T, 2) human K526, 3) rat heart and 4) mouse heart tissue lysate with PA26 antibody. Predicted molecular weight: 50-64 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with PA26 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PA26 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with PA26 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human K526, 3) rat heart and 4) mouse heart tissue lysate with PA26 antibody. Predicted molecular weight: 50-64 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human 293T cells with PA26 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PA26 antibody.

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CodeSizePrice
NSJ-RQ8209-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Sestrin 1, also known as p53-regulated protein PA26 and SEST1, is a protein that in humans is encoded by the SESN1 gene. This gene encodes a member of the sestrin family. Sestrins are induced by the p53 tumor suppressor protein and play a role in the cellular response to DNA damage and oxidative stress. The encoded protein mediates p53 inhibition of cell growth by activating AMP-activated protein kinase, which results in the inhibition of the mammalian target of rapamycin protein. The encoded protein also plays a critical role in antioxidant defense by regenerating overoxidized peroxiredoxins, and the expression of this gene is a potential marker for exposure to radiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-A275) was used as the immunogen for the PA26 antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9Y6P5