NSJ Bioreagents

PYCR2 Antibody / Pyrroline-5-carboxylate reductase 2

Product Code:
 
NSJ-RQ8211
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PYCR2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with PYCR2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human ThP-1, 4) human K562, 5) rat brain, 6) rat thymus, 7) mouse brain and 8) mouse thymus tissue lysate with PYCR2 antibody. Predicted molecular weight ~34 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human JK cells with PYCR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PYCR2 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with PYCR2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human ThP-1, 4) human K562, 5) rat brain, 6) rat thymus, 7) mouse brain and 8) mouse thymus tissue lysate with PYCR2 antibody. Predicted molecular weight ~34 kDa.
Flow cytometry testing of fixed and permeabilized human JK cells with PYCR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PYCR2 antibody.

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CodeSizePrice
NSJ-RQ8211-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Pyrroline-5-carboxylate reductase family, member 2 is a protein that in humans is encoded by the PYCR2 gene. This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q10-D320) was used as the immunogen for the PYCR2 antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
Q96C36