NSJ Bioreagents

NIN Antibody / Ninein

Product Code:
 
NSJ-RQ8214
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Ninein antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Ninein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ninein antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human MOLT4, 3) human MCF7, 4) human U-2 OS, 5) rat heart and 6) mouse heart tissue lysate with Ninein antibody. Predicted molecular weight ~243 (multiple isoforms).
❮❯

Flow cytometry testing of fixed and permeabilized human MCF7 cells with Ninein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ninein antibody.
Western blot testing of 1) human HeLa, 2) human MOLT4, 3) human MCF7, 4) human U-2 OS, 5) rat heart and 6) mouse heart tissue lysate with Ninein antibody. Predicted molecular weight ~243 (multiple isoforms).

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CodeSizePrice
NSJ-RQ8214-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Ninein is a protein that in humans is encoded by the NIN gene. This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q1023-H2090) was used as the immunogen for the Ninein antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q8N4C6