NSJ Bioreagents

SCLY Antibody / Selen°Cysteine lyase

Product Code:
 
NSJ-RQ8215
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Selen°Cysteine lyase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
IHC staining of FFPE human rectum adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE human testicular seminoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human lung adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human tonsil tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE mouse kidney tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE rat kidney tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of human 1) 293T, 2) HepG2, 3) SH-SY5Y and 4) ThP-1 cell lysate with Selenocysteine lyase antibody. Predicted molecular weight ~48 kDa.
8 / 8
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with Selenocysteine lyase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Selenocysteine lyase antibody.
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IHC staining of FFPE human rectum adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular seminoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse kidney tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with Selenocysteine lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) 293T, 2) HepG2, 3) SH-SY5Y and 4) ThP-1 cell lysate with Selenocysteine lyase antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with Selenocysteine lyase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Selenocysteine lyase antibody.

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CodeSizePrice
NSJ-RQ8215-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
SCLY (Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al.(2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E157-A445) was used as the immunogen for the Selenocysteine lyase antibody.
Localisation:
Cytoplasm, Nucleus
Purity:
Antigen affinity purified
Uniprot #:
Q96I15