NSJ Bioreagents

PRR5 Antibody / Proline-rich protein 5 / Protor-1

Product Code:
 
NSJ-RQ8224
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Protor-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human 293T cells with Protor-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protor-1 antibody.
2 / 2
Western blot testing of human 1) 293T, 2) HeLa, 3) Jurkat and 4) HepG2 cell lysate with Protor-1 antibody. Predicted molecular weight: 31-45 kDa (multiple isoforms).
❮❯

Flow cytometry testing of fixed and permeabilized human 293T cells with Protor-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protor-1 antibody.
Western blot testing of human 1) 293T, 2) HeLa, 3) Jurkat and 4) HepG2 cell lysate with Protor-1 antibody. Predicted molecular weight: 31-45 kDa (multiple isoforms).

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CodeSizePrice
NSJ-RQ8224-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes a protein with a proline-rich domain. This gene is located in a region of chromosome 22 reported to contain a tumor suppressor gene that may be involved in breast and colorectal tumorigenesis. The protein is a component of the mammalian target of rapamycin complex 2 (mTORC2), and it regulates platelet-derived growth factor (PDGF) receptor beta expression and PDGF signaling to Akt and S6K1. Alternative splicing and the use of alternative promoters results in transcripts encoding different isoforms. Read-through transcripts from this gene into the downstream Rho GTPase activating protein 8 (ARHGAP8) gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids S45-D366) was used as the immunogen for the Protor-1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P85299