NSJ Bioreagents

PRUNE Antibody / Exopolyphosphatase PRUNE1

Product Code:
 
NSJ-RQ8229
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PRUNE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with PRUNE antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human 293T, 2) human Jurkat, 3) human HEL, 4) human MCF7, 5) rat brain, 6) mouse brain and 7) mouse RAW264.7 cell lysate with PRUNE antibody. Predicted molecular weight ~50 kDa (multiple isoforms) but commonly observed at 50-60 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with PRUNE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRUNE antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with PRUNE antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human Jurkat, 3) human HEL, 4) human MCF7, 5) rat brain, 6) mouse brain and 7) mouse RAW264.7 cell lysate with PRUNE antibody. Predicted molecular weight ~50 kDa (multiple isoforms) but commonly observed at 50-60 kDa.
Flow cytometry testing of fixed and permeabilized human 293T cells with PRUNE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRUNE antibody.

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CodeSizePrice
NSJ-RQ8229-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-Q444) was used as the immunogen for the PRUNE antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q86TP1