NSJ Bioreagents

Recombinant Cdc20 Antibody

Product Code:
 
NSJ-V9429
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rCDC20/7184
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Cdc20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human kidney cancer tissue with recombinant Cdc20 antibody (clone rCDC20/7184) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant Cdc20 antibody (clone rCDC20/7184) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human kidney cancer tissue with recombinant Cdc20 antibody (clone rCDC20/7184) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Cdc20 antibody (clone rCDC20/7184) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9429-20UG20 ug£264.00
Quantity:
NSJ-V9429-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human Cdc20 protein was used as the immunogen for the recombinant Cdc20 antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
Q12834