NSJ Bioreagents

Recombinant CD2 Antibody

Product Code:
 
NSJ-V9463SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LFA2/3417R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the recombinant CD2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 6
Western blot testing of human Jurkat cell lysate using recombinant CD2 antibody (clone LFA2/3417R). Expected molecular weight ~47 kDa.
2 / 6
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human lymph node tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 6
Negative control: IHC staining of FFPE human brain tissue with recombinant CD2 antibody (clone LFA2/3417R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
6 / 6
SDS-PAGE analysis of purified, BSA-free recombinant CD2 antibody (clone LFA2/3417R) as confirmation of integrity and purity.
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Western blot testing of human Jurkat cell lysate using recombinant CD2 antibody (clone LFA2/3417R). Expected molecular weight ~47 kDa.
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD2 antibody (clone LFA2/3417R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node tissue with recombinant CD2 antibody (clone LFA2/3417R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD2 antibody (clone LFA2/3417R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD2 antibody (clone LFA2/3417R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9463SAF-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Description:
CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant protein fragment from the N-terminal region was used as the immunogen for the recombinant CD2 antibody.
Localisation:
Cell surface
Purity:
Protein A/G affinity
Uniprot #:
P06729