NSJ Bioreagents

RHOBTB2 Antibody / DBC2

Product Code:
 
NSJ-V9500
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DBC2/3361
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the RHOBTB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human renal oncocytoma with RHOBTB2 antibody (clone DBC2/3361) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human thyroid tissue with RHOBTB2 antibody (clone DBC2/3361) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
SDS-PAGE analysis of purified, BSA-free RHOBTB2 antibody (clone DBC2/3361) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RHOBTB2 antibody (clone DBC2/3361). These results demonstrate the foremost specificity of the DBC2/3361 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human renal oncocytoma with RHOBTB2 antibody (clone DBC2/3361) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid tissue with RHOBTB2 antibody (clone DBC2/3361) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free RHOBTB2 antibody (clone DBC2/3361) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RHOBTB2 antibody (clone DBC2/3361). These results demonstrate the foremost specificity of the DBC2/3361 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9500-20UG20 ug£264.00
Quantity:
NSJ-V9500-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
The Rho subfamily of Ras-related GTPases controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. DBC-2 (deleted in breast cancer 2 gene protein), also known as RHOBTB2 (Rho-related BTB domain-containing protein 2), is a 727 amino acid member of the RhoBTB subfamily of Rho GTPases. Members of the RhoBTB subfamily are evolutionarily conserved and are characterized by a proline-rich region, a GTPase domain and two tandem BTB repeats. Expressed ubiquitously with highest levels in neural tissue, heart, brain and fetal lung, DBC-2 contains two BTB (POZ) domains through which it may bind to and regulate the function of target proteins, such as CUL-3. Additionally, DBC-2 is thought to function as a regulator of cell cycle and apoptosis events. Under normal conditions, DBC-2 is thought to exhibit tumor suppressor activity. Mutations in the gene encoding DBC-2 are associated with breast cancer, suggesting that mutated DBC-2 may play a role in carcinogenesis.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portin of amino acids 554-604 from the human protein was used as the immunogen for the RHOBTB2 antibody.
Localisation:
Secreted in plasma
Purity:
Protein A/G affinity
Uniprot #:
Q9BYZ6