NSJ Bioreagents

GATA Binding Protein 3 Antibody / GATA-3

Product Code:
 
NSJ-V9502SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
GATA3/6664
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the GATA Binding Protein 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC staining of FFPE human bladder tissue with GATA Binding Protein 3 antibody (clone GATA3/6664). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human lobular breast carcinoma tissue with GATA Binding Protein 3 antibody (clone GATA3/6664). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human lobular breast carcinoma tissue with GATA Binding Protein 3 antibody (clone GATA3/6664). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 5
Negative control: IHC staining of FFPE human brain tissue with GATA Binding Protein 3 antibody (clone GATA3/6664) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
5 / 5
SDS-PAGE analysis of purified, BSA-free GATA Binding Protein 3 antibody (clone GATA3/6664) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human bladder tissue with GATA Binding Protein 3 antibody (clone GATA3/6664). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lobular breast carcinoma tissue with GATA Binding Protein 3 antibody (clone GATA3/6664). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lobular breast carcinoma tissue with GATA Binding Protein 3 antibody (clone GATA3/6664). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with GATA Binding Protein 3 antibody (clone GATA3/6664) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free GATA Binding Protein 3 antibody (clone GATA3/6664) as confirmation of integrity and purity.

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NSJ-V9502SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the GATA Binding Protein 3 antibody.
Localisation:
Nuclear
Purity:
Protein A/G affinity
Uniprot #:
P23771