NSJ Bioreagents

Surfactant protein D Antibody / SFTPD

Product Code:
 
NSJ-V9563
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SFTPD/4363
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Surfactant protein D antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human lung tissue with Surfactant Protein D antibody (clone SFTPD/4363). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human lung tissue with Surfactant Protein D antibody (clone SFTPD/4363). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
SDS-PAGE analysis of purified, BSA-free Surfactant protein D antibody (clone SFTPD/4363) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Surfactant protein D antibody (clone SFTPD/4363). These results demonstrate the foremost specificity of the SFTPD/4363 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC staining of FFPE human lung tissue with Surfactant Protein D antibody (clone SFTPD/4363). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung tissue with Surfactant Protein D antibody (clone SFTPD/4363). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Surfactant protein D antibody (clone SFTPD/4363) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Surfactant protein D antibody (clone SFTPD/4363). These results demonstrate the foremost specificity of the SFTPD/4363 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9563-20UG20 ug£264.00
Quantity:
NSJ-V9563-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C, SP-D. SP-B and SP-C are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A and SP-D are large multimeric proteins belonging to the family of calcium-dependent lectins, designated Collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 241-336 was used as the immunogen for the Surfactant protein D antibody.
Localisation:
Secreted
Purity:
Protein A/G affinity
Uniprot #:
P35247