NSJ Bioreagents

Recombinant KRT7 Antibody / Cytokeratin 7

Product Code:
 
NSJ-V9566SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KRT7/4387R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant KRT7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human salivary gland tissue with recombinant KRT7 antibody (clone KRT7/4387R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human ovarian carcinoma tissue with recombinant KRT7 antibody (clone KRT7/4387R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of FFPE human bladder carcinoma tissue with recombinant KRT7 antibody (clone KRT7/4387R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
4 / 4
Negative control: IHC staining of FFPE human brain tissue with recombinant KRT7 antibody (clone KRT7/4387R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human salivary gland tissue with recombinant KRT7 antibody (clone KRT7/4387R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian carcinoma tissue with recombinant KRT7 antibody (clone KRT7/4387R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder carcinoma tissue with recombinant KRT7 antibody (clone KRT7/4387R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant KRT7 antibody (clone KRT7/4387R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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NSJ-V9566SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
Cytokeratin 7 in humans is a protein encoded by the KRT7 gene. It is always as a keratin pair with Cytokeratin 19. Cytokeratin 7 is expressed on most ductal and glandular epithelium including lung, breast, bladder and female genital tract, but not in most gastrointestinal epithelium, prostate, hepatocyte and squamous epithelium. In tumor tissues, its expression is absent in colon cancer, prostate cancer and squamous carcinomas. Cytokeratin 7 is often used in concert with Cytokeratin 20 and CDX-2 to aid in distinguishing ovarian, pulmonary and breast carcinomas (CK7+) from colon carcinomas (CK7-).
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 1-100 was used as the immunogen for the recombinant KRT7 antibody.
Localisation:
Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P08729