NSJ Bioreagents

SPARC Antibody / Osteonectin

Product Code:
 
NSJ-V9574SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
OSTN/3933
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the SPARC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human placental tissue with SPARC antibody (clone OSTN/3933). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human tonsil tissue with SPARC antibody (clone OSTN/3933). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
SDS-PAGE analysis of purified, BSA-free SPARC antibody (clone OSTN/3933) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPARC antibody (clone OSTN/3933). These results demonstrate the foremost specificity of the OSTN/3933 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

IHC staining of FFPE human placental tissue with SPARC antibody (clone OSTN/3933). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with SPARC antibody (clone OSTN/3933). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free SPARC antibody (clone OSTN/3933) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPARC antibody (clone OSTN/3933). These results demonstrate the foremost specificity of the OSTN/3933 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9574SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 1-200 was used as the immunogen for the SPARC antibody.
Localisation:
Secreted
Purity:
Protein A/G affinity
Uniprot #:
P09486