NSJ Bioreagents

Recombinant HSP27 Antibody / HSPB1

Product Code:
 
NSJ-V9625
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
HSPB1/7038R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant HSP27 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human prostate tissue with recombinant HSP27 antibody (clone HSPB1/7038R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/7038R) as confirmation of integrity and purity.

IHC staining of FFPE human prostate tissue with recombinant HSP27 antibody (clone HSPB1/7038R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/7038R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V9625-20UG20 ug£264.00
Quantity:
NSJ-V9625-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml
Description:
HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant human full-length protein was used as the immunogen for the recombinant HSP27 antibody.
Localisation:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P04792