NSJ Bioreagents

Neurogenin 3 Antibody / NGN3

Product Code:
 
NSJ-V9690SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-NEUROG3-1E10
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the Neurogenin 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using Neurogenin 3 antibody (green, clone PCRP-NEUROG3-1E10) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with Neurogenin 3 antibody (blue, clone PCRP-NEUROG3-1E10) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free Neurogenin 3 antibody (clone PCRP-NEUROG3-1E10) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Neurogenin 3 antibody (clone PCRP-NEUROG3-1E10). These results demonstrate the foremost specificity of the PCRP-NEUROG3-1E10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using Neurogenin 3 antibody (green, clone PCRP-NEUROG3-1E10) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with Neurogenin 3 antibody (blue, clone PCRP-NEUROG3-1E10) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free Neurogenin 3 antibody (clone PCRP-NEUROG3-1E10) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Neurogenin 3 antibody (clone PCRP-NEUROG3-1E10). These results demonstrate the foremost specificity of the PCRP-NEUROG3-1E10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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NSJ-V9690SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Description:
This antigen is a transcription factor that interacts with NKX2-2 to regulation transcription of NEUROD1. The neurogenin family of proteins belongs to the basic helix-loop-helix (bHLH) superfamily and consists of Neurogenin 1, Neurogenin 2 and Neurogenin 3 (also designated ngn3). bHLH members are transcriptional regulators that determine cell fate. Neurogenin 3 is expressed in discrete regions of developing neurons and in the embryonic pancreatic islets of Langerhans. HNF-6 (hepatocyte nuclear factor 6) acts as a positive regulator of Neurogenin 3 by binding to and stimulating the neurogenin gene promoter. Neurogenin 3 is involved in the initial differentiation of the four islets cell types, while a network of transcription factors, including Hlxb9, Isl1, NeuroD, Nkx-2.2, Nkx-6.4, Pax-4, Pax-6, PDX-1 and Mash1, are required for final differentiation. Neurogenin 3 acts upstream of NeuroD in a bHLH cascade. Neurogenin 3 activates the expression of NeuroD at the onset of islet cell differentiation through box sequences E1 and E3 in the NeuroD promoter.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human full-length protein was used as the immunogen for the Neurogenin 3 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q9Y4Z2