NSJ Bioreagents

PHF10 Antibody

Product Code:
 
NSJ-V9695
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-PHF10-2A10
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the PHF10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using PHF10 antibody (green, clone PCRP-PHF10-2A10) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with PHF10 antibody (blue, clone PCRP-PHF10-2A10) and isotype control (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free PHF10 antibody (clone PCRP-PHF10-2A10) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PHF10 antibody (clone PCRP-PHF10-2A10). These results demonstrate the foremost specificity of the PCRP-PHF10-2A10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using PHF10 antibody (green, clone PCRP-PHF10-2A10) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with PHF10 antibody (blue, clone PCRP-PHF10-2A10) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free PHF10 antibody (clone PCRP-PHF10-2A10) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using PHF10 antibody (clone PCRP-PHF10-2A10). These results demonstrate the foremost specificity of the PCRP-PHF10-2A10 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9695-20UG20 ug£264.00
Quantity:
NSJ-V9695-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Description:
This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human PHF10 protein was used as the immunogen for the PHF10 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q8WUB8