NSJ Bioreagents

ERK2 Antibody / MAPK1

Product Code:
 
NSJ-V9723
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-MAPK1-1D1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the ERK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using ERK2 antibody (green, clone PCRP-MAPK1-1D1) and phalloidin (red).
2 / 4
FACS staining of PFA-fixed human HeLa cells with ERK2 antibody (blue, clone PCRP-MAPK1-1D1), and unstained cells (red).
3 / 4
SDS-PAGE analysis of purified, BSA-free ERK2 antibody (clone PCRP-MAPK1-1D1) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ERK2 antibody (clone PCRP-MAPK1-1D1). These results demonstrate the foremost specificity of the PCRP-MAPK1-1D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

Immunofluorescent staining of PFA-fixed human HeLa cells using ERK2 antibody (green, clone PCRP-MAPK1-1D1) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ERK2 antibody (blue, clone PCRP-MAPK1-1D1), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free ERK2 antibody (clone PCRP-MAPK1-1D1) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ERK2 antibody (clone PCRP-MAPK1-1D1). These results demonstrate the foremost specificity of the PCRP-MAPK1-1D1 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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CodeSizePrice
NSJ-V9723-20UG20 ug£264.00
Quantity:
NSJ-V9723-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Flow cytometry: 0.5-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Recombinant full-length human protein was used as the immunogen for the ERK2 antibody.
Localisation:
Cytoplasm, Nucleus
Purity:
Protein A/G affinity
Uniprot #:
P28482