NSJ Bioreagents

MED22 Antibody

Product Code:
 
NSJ-V9732SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-MED22-1E4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Aliquot the MED22 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MED22 antibody (clone PCRP-MED22-1E4). These results demonstrate the foremost specificity of the PCRP-MED22-1E4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
FACS staining of PFA-fixed human HeLa cells with MED22 antibody (blue, clone PCRP-MED22-1E4), and unstained cells (red).
3 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using MED22 antibody (green, clone PCRP-MED22-1E4) and phalloidin (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free MED22 antibody (clone PCRP-MED22-1E4) as confirmation of integrity and purity.
❮❯

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MED22 antibody (clone PCRP-MED22-1E4). These results demonstrate the foremost specificity of the PCRP-MED22-1E4 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with MED22 antibody (blue, clone PCRP-MED22-1E4), and unstained cells (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using MED22 antibody (green, clone PCRP-MED22-1E4) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free MED22 antibody (clone PCRP-MED22-1E4) as confirmation of integrity and purity.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V9732SAF-100UG100 ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
ELISA (order BSA-free format for coating),Immunofluorescence: 0.5-2ug/ml,Flow cytometry: 0.5-2ug/million cells,Western blot: 1-2ug/ml
Description:
Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (mediator complex subunit 22), also known as SURF5 (surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the MED22 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
Q15528