NSJ Bioreagents

MED7 Antibody

Product Code:
 
NSJ-V9748SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PCRP-MED7-1B8
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Aliquot the MED7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MED7 antibody (clone PCRP-MED7-1B8). These results demonstrate the foremost specificity of the PCRP-MED7-1B8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
2 / 4
FACS staining of PFA-fixed human HeLa cells with MED7 antibody (blue, clone PCRP-MED7-1B8) and isotype control (red).
3 / 4
Immunofluorescent staining of PFA-fixed human HeLa cells using MED7 antibody (green, clone PCRP-MED7-1B8) and phalloidin (red).
4 / 4
SDS-PAGE analysis of purified, BSA-free MED7 antibody (clone PCRP-MED7-1B8) as confirmation of integrity and purity.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MED7 antibody (clone PCRP-MED7-1B8). These results demonstrate the foremost specificity of the PCRP-MED7-1B8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with MED7 antibody (blue, clone PCRP-MED7-1B8) and isotype control (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using MED7 antibody (green, clone PCRP-MED7-1B8) and phalloidin (red).
SDS-PAGE analysis of purified, BSA-free MED7 antibody (clone PCRP-MED7-1B8) as confirmation of integrity and purity.

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NSJ-V9748SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (use assay dependent concentration,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
Description:
MED7 (Mediator of RNA polymerase II transcription subunit 7), also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, MED7 functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5. Additionally, MED7 regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. MED7 may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases MED7 mRNA-binding affinity. Defects or deletions in the gene encoding MED7 are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of MED7 exist due to alternative splicing events.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human protein was used as the immunogen for the MED7 antibody.
Localisation:
Nucleus
Purity:
Protein A/G affinity
Uniprot #:
O43513