Abnova Corporation

SMAD3 (phospho T179) polyclonal antibody

Product Code:
 
PAB19947
Product Group:
 
Primary Antibodies
Supplier:
 
Abnova Corporation
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
 

No additional charges, what you see is what you pay! *

CodeSizePrice
PAB19947-100ug100 ug£709.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Taiwan.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396
Gene Description:
SMAD family member 3
Gene ID:
4088
Gene Name:
SMAD3
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding T179 of human SMAD3.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product Description:
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.
Recommend Dilutions:
ELISA (1:15000-1:75000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
Specificity:
This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pT179 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad2. Reactivity against other phosphorylated Smad family members is not known. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, mouse, rat, pig, dog, and marmoset based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Storage Buffer:
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)