MYD88 Antibody
PSI-2125
Rabbit
IgG
Polyclonal
RUO
- Human
- Mouse
- Rat
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
No additional charges, what you see is what you pay! *
Code | Size | Price |
---|
PSI-2125-0.02mg | 0.02mg | £150.00 |
PSI-2125-0.1mg | 0.1mg | £449.00 |
* Rare exceptions are clearly labelled (only 0.14% of items!).
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- Further Information
- Documents
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Further Information
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry, Immunoprecipitation and Immunofluorescence in human samples. All other applications and species not yet tested.
- Medzhitov et al. Mol. Cell. 1998;2:253-8
- Kawai et al. Nat. Immunol. 2004;5:1061-8
- Semaan et al. J. Immunol. 2008;180:3485-91
- Muzio et al. Science 1997;278:1612-5
- Yamamoto et al. Mol. Immunol. 2014;58:66-76
- Ohnishi et al. Proc. Natl. Acad. Sci. U.S.A. 2009;106:10260-5
- Adachi et al. Immunity 1998;9:143-50
- Kawai et al. Immunity 1999;11:115-22
The immunogen is located within amino acids 220 - 270 of MYD88.
Observed: 35kD
Independent Antibody Validation in Cell lines (Figure 2) shows similar MYD88 expression profile in both human and mouse cell lines detected by two independent anti-MYD88 antibodies that recognize different epitopes, 2125 against internal domain and 2127 against the C-terminus domain. MYD88 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Additionally, Figure 2 shows the mouse MYD88 protein in NIH/3T3 cells migrates slightly faster than human isoform 1 detected by both MYD88 antibodies (2125 and 2127), which is well correlated with their calculated molecular masses (33.8 kDa vs 35.4 kDa).
Independent Antibody Validation in Human Tissues (Figure 3) shows similar MYD88 expression profile in human tissues detected by two independent anti-MYD88 antibodies (2125 and 2127). MYD88 proteins are detected by the two independent antibodies in liver, kidney, lung, thymus, colon, bladder and breast of human tissues at different expression levels, but not in heart, brain, skin and pancreas.
Animal Species Reactivity (Figure 4): Anti-MYD88 antibodies (2125 and 2127) can detect the expression of MYD88 protein in the liver and spleen of all tissues and mouse heart, but not in human heart. Additionally, Figure 4 also shows MYD88 protein detected by both MYD88 antibodies (2125 and 2127) in human liver and Daudi cells migrates slightly slower than that in the tissues of mouse and rat, which is well correlated with their calculated molecular masses (35.4 kDa vs 33.8 kDa and 33.9kD).
siRNA knockdown validation (Figure 5): Anti-MYD88 antibody (2125) specificity was further verified by MYD88 specific siRNA knockdown. MYD88 signal in HeLa cells transfected with MYD88 siRNAs was weaker in comparison with that in HeLa cells transfected with control siRNAs.
Documents
References
- Miller et al. MyD88 media16413925tes neutrophil recruitment initiated by IL-1R but not TLR2 activation in immunity against Staphylococcus aureus. Immunity. 2006;24(1):79-91. PMID: 16413925
- Burns et al. Inhibition of Interleukin 1 Receptor/Toll-like Receptor Signaling through the Alternatively Spliced, Short Form of MyD88 Is Due to Its Failure to Recruit IRAK-4. J Exp Med. 2003;197(2): 263-8. PMID: 12538665
- Vlantis et al. TLR-independent anti-inflammatory function of intestinal epithelial TRAF6 signalling prevents DSS-induced colitis in mice. Gut. 2016;65(6):935-43.PMID: 25761602
- Ahmad et al. Elevated expression of the toll like receptors 2 and 4 in obese individuals: its significance for obesity-induced inflammation. J Inflamm (Lond). 2012;9:48. PMID: 23191980
- Kawai et al. Interferon-alpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6. Nat Immunol. 2004;5(10):1061-8.PMID: 15361868
- Romics L Jr et al. Selective priming to Toll-like receptor 4 (TLR4), not TLR2, ligands by P. acnes involves up-regulation of MD-2 in mice. Hepatology. 2004;40(3):555-64PMID: 15349893
- Ahmad et al. Requirement of TLR2-mediated signaling for the induction of IL-15 gene expression in human monocytic cells by HSV-1. Blood. 2008;112(6):2360-8. PMID: 18583567
- Kawagoe T et al. Essential role of IRAK-4 protein and its kinase activity in Toll-like receptor-mediated immune responses but not in TCR signaling. J Exp Med. 2007;204(5):1013-24. PMID: 17485511
- Lin et al. The tyrosine kinase Syk differentially regulates Toll-like receptor signaling downstream of the adaptor molecules TRAF6 and TRAF3. Sci Signal. 2013;6(289):ra71. PMID: 23962979
- Ahmad et al. MyD88, IRAK1 and TRAF6 knockdown in human chondrocytes inhibits interleukin-1-induced matrix metalloproteinase-13 gene expression and promoter activity by impairing MAP kinase activation. Cell Signal. 2007;19(12):2549-57. PMID: 17905570
- Mukherjee et al. Lipopolysaccharide-driven Th2 Cytokine Production in Macrophages Is Regulated by Both MyD88 and TRAM. J Biol Chem. 2009;284(43): 29391-8.PMID: 19638630
- Campanholle et al. TLR-2/TLR-4 TREM-1 Signaling Pathway Is Dispensable in Inflammatory Myeloid Cells during Sterile Kidney Injury. PLoS One. 2013;8(7): e68640. PMID: 23844229
- Murakami et al. Human herpesvirus 6 infection impairs Toll-like receptor signaling. Virol J. 2010;7:91.PMID: 20459723
- Su et al. Expression and regulation of Toll-like receptor 2 by IL-1beta and fibronectin fragments in human articular chondrocytes. Osteoarthritis Cartilage. 2005;13(10):879-86. PMID: 15961329
- Pedrosa et al. Bacteria and spontaneous experimental colitis: immunological changes. Eur J Clin Invest. 2011;41(10):1047-53. PMID: 21902690
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