Further Information
Slug Antibody: SLUG, WS2D, SLUGH1, SNAIL2, SLUG, SLUGH, Zinc finger protein SNAI2, Neural crest transcription factor Slug
Slug antibody can be used for detection of Slug by Western blot at 1 to 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Slug Antibody: Slug, a member of the Snail family of C2H2-type zinc finger transcription factors, was initially identified to be involved in epithelial-mesenchymal transitions as well as the formation of the neural tube during vertebrate embryogenesis. Like Snail, Slug transcription can be induced by growth factors such as FGF, BMP, and TGF-beta. Once expressed, Slug will bind E-box regions of promoters and repress transcription of genes such as E-cadherin and Claudin-1. More recently, its expression in breast, esophogeal, and colorectal carcinomas has been correlated with poor prognosis for survival. Furthermore, Slug can protect hemapoietic progenitor cells from radiation-induced apoptosis by repressing the p53-mediated transcription of Puma, a BH3-only antagonist of the anti-apoptotic members of the Bcl-2 family. Slug antibody has no cross-reactivity to Snail protein.
- Neito MA, Sargent MG, Wilkinson DG, et al. Control of cell behavior during vertebrate development by Slug, a zinc finger gene. Science 1994; 264:835-9.
- Martinez-Estrada OM, Culleres A, Soriano FX, et al. The transcription factors Slug and Snail act as repressors of Claudin-1 expression in epithelial cells. Biochim J. 2006; 394:449-57.
- Shioiri M, Shida T, Koda K, et al. Slug expression is an independent prognostic parameter for poor survival in colorectal carcinoma patients. Br. J. Cancer 2006; 94:1816-22.
- Wu WS, Heinrichs S, Xu D, et al. Slug antagonizes p53-mediated apoptosis of hematopoietic progenitors by repressing puma. Cell 2005; 123:641-53.
Slug Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%)
Slug antibody was raised against a 14 amino acid synthetic peptide from near the center of human Slug.
The immunogen is located within amino acids 70 - 120 of Slug.
6591
snail homolog 2 (Drosophila)
SNAI2
Homo sapiens
Liquid
O43623
11134406
Slug Antibody is affinity chromatography purified via peptide column.
Signal Transduction,Cancer
O43623
Optimal dilutions for each application to be determined by the researcher.