ProSci

SCO2 Antibody

Product Code:
 
PSI-4045
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of SCO2 in HL60 cell lysate lysate with SCO2 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of SCO2 in human liver tissue with SCO2 antibody at 2.5 μg/mL.
3 / 3
Immunofluorescence of SCO2 in Human Liver tissue with SCO2 antibody at 20 μg/mL.
❮❯

Western blot analysis of SCO2 in HL60 cell lysate lysate with SCO2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of SCO2 in human liver tissue with SCO2 antibody at 2.5 μg/mL.
Immunofluorescence of SCO2 in Human Liver tissue with SCO2 antibody at 20 μg/mL.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-4045-0.02mg0.02mg£150.00
Quantity:
PSI-4045-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Additional Names:
SCO2 Antibody: SCOD1, Protein SCO2 homolog, mitochondrial
Application Note:
SCO2 antibody can be used for detection of SCO2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SCO2 Antibody: Synthesis of cytochrome c oxidase 2 was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO2 and SCO1 are dependent on copper ion binding. Recent studies suggest that SCO2 expression is regulated by p53, so that a decrease in p53 expression, such as in numerous tumors and cells lines, the drop in SCO2 expression leads to a shift from normal aerobic respiration towards the production of glycolytic ATP. Defects in the SCO2 protein are also associated with fatal infantile cardioencephalomyopathy and COX deficiency.
Background References:
  • Glerum DM, Shtanko A, and Tzagoloff A. SCO1 and SCO2 act as high copy suppressors of a mitochondrial copper recruitment defect in Saccharomyces cerevisiae. J. Biol. Chem. 1996; 271:20531-5.
  • Horng Y-C, Leary SC, Cobine PA, et al. Human Sco1 and Sco2 function as copper-binding proteins. J. Biol. Chem. 2005; 280:34113-22.
  • Matoba S, Kang J-G, Patino WD, et al. p53 regulates mitochondrial respiration. Science 2006; 312:1650-3.
  • Papadopoulou LC, Sue CM, Davidson MM, et al. Fatal infantile cardioencephalomyopathy with COX deficiency and mutations in SCO2, a COX assembly gene. Nat. Genetics 1999; 23:333-7.
Buffer:
SCO2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (84%), Bovine: (84%)
Immunogen:
SCO2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human SCO2.

The immunogen is located within the last 50 amino acids of SCO2.
NCBI Gene ID #:
9997
NCBI Official Name:
cytochrome c oxidase assembly protein
NCBI Official Symbol:
SCO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 29 kDa

Observed: 33 kDa
Protein Accession #:
O43819
Protein GI Number:
8134662
Purification:
SCO2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O43819
User NOte:
Optimal dilutions for each application to be determined by the researcher.