Further Information
ORAI2 Antibody: CBCIP2, C7orf19, MEM142B, TMEM142B, CBCIP2, PP1729, Protein orai-2, CAP-binding protein complex-interacting protein 2
ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
ORAI2 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1.
- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
- Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.
ORAI2 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
ORAI2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2.
The immunogen is located within the last 50 amino acids of ORAI2.
80228
ORAI calcium release-activated calcium modulator 2
ORAI2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 19, 28 kDa
Observed: 29 kDa
Q96SN7
74732728
ORAI2 Antibody is affinity chromatography purified via peptide column.
Immunology
At least two isoforms of ORAI2 are known to exist; this antibody will detect both isoforms. ORAI antibody is predicted not to cross-react with ORAI1 or ORAI3.
Q96SN7
Optimal dilutions for each application to be determined by the researcher.