ProSci

TBC1D1 Antibody

Product Code:
 
PSI-4231
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
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Western blot analysis of TBC1D1 expression in (A) 293 and (B) Daudi cell lysate with TBC1D1 antibody at ?2 μg/ml.
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Western blot analysis of TBC1D1 expression in (A) 293 and (B) Daudi cell lysate with TBC1D1 antibody at ?2 μg/ml.

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CodeSizePrice
PSI-4231-0.02mg0.02mg£150.00
Quantity:
PSI-4231-0.1mg0.1mg£449.00
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Further Information

Additional Names:
TBC1D1 Antibody: TBC, TBC1, KIAA1108, TBC1 domain family member 1
Application Note:
TBC1D1 antibody can be used for detection of TBC1D1 by Western blot at 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
TBC1D1 Antibody: TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain and presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2/USP6, yeast Bub2, and CDC16. TBC1D1 and TBC1D4 (AS160) have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. TBC1D1 regulates insulin-mediated GLUT4 translocation through its GAP activity, and is a likely Akt substrate. Mutations in the Tbc1d1 gene lead to some cases of severe human obesity.
Background References:
  • White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet.2000; 89:272-5.
  • Koumanov F and Holman GD. Thrifty Tbc1d1 and Tbc1d4 proteins link signalling and membrane trafficking pathways. Biochem. J.2007; 403:e9-11.
  • Roach WG, Chavez JA, Miinea CP, et al. Substrate specificity and effect on GLUT4 translocation of the Rab GTPase-activating protein Tbc1d1. Biochem J.2007; 403:353-8.
  • Stone S, Abkevich V, Russell DL, et al. TBC1D1 is a candidate for a severe obesity gene and evidence for a gene/gene interaction in obesity predisposition. Hum. Mol. Genet.2006; 15:2709-20.
Buffer:
TBC1D1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (71%)
Immunogen:
TBC1D1 antibody was raised against a 22 amino acid synthetic peptide from near the carboxy terminus of human TBC1D1.

The immunogen is located within the last 50 amino acids of TBC1D1.
NCBI Gene ID #:
23216
NCBI Official Name:
TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
NCBI Official Symbol:
TBC1D1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 128 kDa

Observed: 140 kDa?
Protein Accession #:
NP_055988
Protein GI Number:
50658061
Purification:
TBC1D1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Signal Transduction,Cancer
Swissprot #:
Q86TI0
User NOte:
Optimal dilutions for each application to be determined by the researcher.