Further Information
NPTX2 Antibody: NP2, NARP, NP-II, Neuronal pentraxin-2, Neuronal pentraxin II, NP2
WB: 0.5-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
NPTX2 Antibody: Neuronal pentraxin (NPTX) 2 was initially identified as a member of the pentraxin family having high homology to NPTX1. Unlike NPTX1 however, NPTX2 is expressed in testes, pancreas, liver, heart, skeletal muscle as well as brain. NPTX2 possesses the lectin properties common to the pentraxin family, promotes neurite outgrowth, and is rapidly regulated by neuronal activity. NPTX1 and NPTX2 form heterocomplexes that contribute to both activity-independent and -dependent excitatory synaptogenesis. Recently, NPTX2 has been found to be highly upregulated in Parkinsonian substantia nigra and localizes to Lewy bodies and Lewy neurites in sporadic Parkinson's disease (PD), suggesting that it is involved in the pathway dysregulation that underlies PD. This NPTX2 antibody is predicted to be specific to NPTX2 and not recognize NPTX1.
- Hsu and Perin. Genomics1995; 28:220-7.
- Tsu et al. J. Neurosci.1996; 16:2463-78.
- Xu et al. Neuron2003; 39:513-28.
- Moran et al. Acta Neuropathol.2008; 115:471-8.
NPTX2 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Guinea pig: (88%)
Anti-NPTX2 antibody (4573) was raised against a peptide corresponding to 16 amino acids near the center of human NPTX2.
The immunogen is located within amino acids 170 - 220 of NPTX2.
Human NPTX2 has one isoform (431aa, 47kD). Mouse NPTX2 has one isoform (429aa, 47.1kD) and Rat NPTX2 also has one isoform (432aa, 47.4kD). 4573 can detect human, mouse and rat.
4885
neuronal pentraxin II
NPTX2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 47kD
Observed: 47 kD
NP_002514
28195384
NPTX2 Antibody is affinity chromatography purified via peptide column.
Neuroscience
P47972
Optimal dilutions for each application to be determined by the researcher.
Independent Antibody Validation in Cell lines (Figure 2) shows similar NPTX2 expression profile in human and mouse cell lines detected by two independent anti-NPTX2 antibodies that recognize different epitopes, 4573 against central domain and 64-234 against another central domain. NPTX2 proteins are detected only in HeLa and HepG2 cell lines by the two independent antibodies.