Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
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![Western blot analysis of Wnt10b in human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 μg/mL. Western blot analysis of Wnt10b in human skeletal muscle tissue lysate with Wnt10b antibody at (A) 2 and (B) 4 μg/mL.](https://www.prosci-inc.com/static-images/4619oem.jpg)
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![Immunohistochemistry of Wnt10b in human skeletal muscle with Wnt10b antibody at 2.5 μg/mL. Immunohistochemistry of Wnt10b in human skeletal muscle with Wnt10b antibody at 2.5 μg/mL.](https://www.prosci-inc.com/static-images/Wnt10b-Antibody_IHC_4619.jpg)
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Further Information
Wnt10b Antibody: SHFM6, WNT-12, WNT12, Protein Wnt-10b, Protein Wnt-12
Wnt10b antibody can be used for detection of Wnt10b by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Wnt10b Antibody: Wnt10b is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation. Wnt10b is found in the mouse mammary tumor virus insertion site where it is activated and causes mammary tumors. Elevated levels of Wnt10b have also been detected in human breast carcinomas. Wnt10b is known to be involved in adipogenesis, maintaining the preadipocyte in an undifferentiated state. More recently, Wnt10b has been shown to promote epithelial cell differentiation and hair shaft growth, demonstrating that Wnt10b has multiple roles in cell growth and differentiation.
- Lee FS, Lane TF, Kuo A, et al. Insertional mutagenesis identifies a member of the Wnt gene family as a candidate oncogene in the mammary epithelium of int-2/Fgf-3 transgenic mice. Proc. Natl. Acad. Sci. USA1995; 92:2268-72.
- Nusse R and Varmus RE. Wnt genes. Cell1992; 69:1073-87.
- Bui TD, Rankin J, Smith K, et al. A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas. Oncogene1997; 14:1249-53.
- Bennett CN, Hodge CL, MacDougald OA, et al. Role of Wnt10b and C/EBPalpha in spontaneous adipogenesis of 243 cells. Biochem. Biophys. Res. Commun.2003; 302:12-16.
Wnt10b Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b.
The immunogen is located within amino acids 130 - 180 of Wnt10b.
7480
wingless-type MMTV integration site family, member 10B
WNT10B
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 43 kDa
Observed: 47 kDa
NP_003385
16936522
Wnt10b Antibody is affinity chromatography purified via peptide column.
Signal Transduction
Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.
O00744
Optimal dilutions for each application to be determined by the researcher.