Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
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Further Information
SPATA3 Antibody: TSARG1, TSARG1, Spermatogenesis-associated protein 3, Testis and spermatogenesis cell-related protein 1
SPATA3 antibody can be used for detection of SPATA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPATA3 Antibody: The testis and spermatogenesis cell apoptosis related gene 1 (TSARG1) protein, also known as SPATA3, is highly expressed in testis, and to a lower extent in other tissues. SPATA3 contains a highly conserved J domain, which is present in all DnaJ-like proteins, and is thus a novel member of the HSP40 protein family. SPATA3 is thought to be involved in apoptosis of spermatogenic cells.
- Fu JJ, Lu GX, Li LY, et al. Molecular cloning for testis spermatogenesis cell apoptosis related gene TSARG1 and Mtsarg1 and expression analysis for Mtsarg1 gene. Yi Chuan Xue Bao 2003; 30:25-9.
- Yang HM, Liu G, Nie ZY, et al. Molecular cloning of a novel rat gene Tsarg1, a member of the DnaJ/HSP40 protein family. DNA Seq. 2005; 16:166-72.
SPATA3 Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
SPATA3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPATA3.
The immunogen is located within the last 50 amino acids of SPATA3.
130560
spermatogenesis associated 3
SPATA3
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 21 kDa
Observed: 26 kDa
NP_620712
31712020
SPATA3 Antibody is affinity chromatography purified via peptide column.
Apoptosis
At least three isoforms of SPATA3 are known to exist; this antibody will detect only the longest two isoforms. SPATA3 antibody is predicted to not cross-react with SPATA protein family members
Q8NHX4
Optimal dilutions for each application to be determined by the researcher.