ProSci

APBA1 Antibody

Product Code:
 
PSI-6925
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of APBA1 in rat brain tissue lysate with APBA1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunofluorescence of APBA1 in human brain tissue with APBA1 antibody at 20 μg/mL.
❮❯

Western blot analysis of APBA1 in rat brain tissue lysate with APBA1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of APBA1 in human brain tissue with APBA1 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6925-0.02mg0.02mg£150.00
Quantity:
PSI-6925-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
APBA1 Antibody: X11, X11A, LIN10, MINT1, D9S411E, X11ALPHA, X11, Amyloid beta A4 precursor protein-binding family A member 1, Adapter protein X11alpha, Mint-1
Application Note:
APBA1 antibody can be used for detection of APBA1 by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
APBA1 Antibody: The Amyloid beta 4 (A4) precursor protein-binding family A member 1 (APBA1) is a member of the X11 protein family and a neuronal adapter protein that interacts with the Alzheimer's disease amyloid precursor protein (APP). APBA1 stabilizes APP and inhibits production of proteolytic APP fragments including the Aβ peptide that is deposited in the brains of Alzheimer's disease patients. It is thought to be involved in signal transduction processes and is a putative vesicular trafficking protein in the brain that can form a complex with the potential to couple synaptic vesicle exocytosis to neuronal cell adhesion. APBA1 can also bind to CASK, a multidomain scaffolding protein involved in brain development and synapse formation.
Background References:
  • Borg JP, Ooi J, Levy E, et al. The phosphotyrosine interaction domains of X11 and FE65 bind to distinct sites on the YENPTY motif of amyloid precursor protein. Mol. Cell. Biol. 1996; 16:6229-41.
  • Mueller HT, Borg JP, Margolis B, et al. Modulation of amyloid precursor protein metabolism by X11alpha/Mint1. A deletion analysis of protein-protein interaction domains. J. Biol. Chem. 2000; 275:39302-6.
  • Maximov A, Sudhof TC, and Bezprozvanny I. Association of neuronal calcium channels with modular adaptor proteins. J. Biol. Chem. 1999; 274:24453-6
  • Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.
Buffer:
APBA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APBA1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human APBA1.

The immunogen is located within amino acids 90 - 140 of APBA1.
NCBI Gene ID #:
320
NCBI Official Name:
amyloid beta A4 precursor protein-binding family A member 1
NCBI Official Symbol:
APBA1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 92 kDa

Observed: 92 kDa
Protein Accession #:
NP_001154
Protein GI Number:
22035548
Purification:
APBA1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other APBA proteins.
Swissprot #:
Q02410
User NOte:
Optimal dilutions for each application to be determined by the researcher.