ProSci

Fat Free Antibody

Product Code:
 
PSI-6979
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
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Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.
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Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.

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CodeSizePrice
PSI-6979-0.02mg0.02mg£150.00
Quantity:
PSI-6979-0.1mg0.1mg£449.00
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Further Information

Additional Names:
Fat Free Antibody: FFR, ANG2, ANG3, C11orf2, C11orf3, FFR, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein
Application Note:
Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.
Background References:
  • Perez-Victoria FJ, Schindler C, Magadan JG, et al. Ang2/Fat Free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol. Biol. Cell 2010; 21:3386-95
  • Ho SY, Lorent K, Pack M, et al. Zebrafish Fat Free is required for intestinal lipid absorption and Golgi apparatus structure. Cell Metab. 2006; 3:289-300.
  • Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell 2008; 19:2350-62
  • Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.
Buffer:
Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (82%)
Immunogen:
Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free.

The immunogen is located within amino acids 620 - 670 of Fat Free.
NCBI Gene ID #:
738
NCBI Official Name:
chromosome 11 open reading frame 2
NCBI Official Symbol:
C11orf2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
86 kDa
Protein Accession #:
NP_037397
Protein GI Number:
8393009
Purification:
Fat Free Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q9UID3
User NOte:
Optimal dilutions for each application to be determined by the researcher.