ProSci

BCAR3 Antibody

Product Code:
 
PSI-6999
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.
2 / 2
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 μg/mL.
❮❯

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 μg/mL.

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CodeSizePrice
PSI-6999-0.02mg0.02mg£150.00
Quantity:
PSI-6999-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
BCAR3 Antibody: NSP2, SH2D3B, NSP2, UNQ271/PRO308, Breast cancer anti-estrogen resistance protein 3, Novel SH2-containing protein 2
Application Note:
BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
BCAR3 Antibody: Breast cancer anti-estrogen resistance 3 (BCAR3) was identified in the search for genes involved in the development of estrogen resistance. It contains a putative src homology 2 (SH2) domain and is partly homologous to the cell division cycle protein CDC48. BCAR3 is a binding partner with Cas, an adapter molecule that plays a role in cell proliferation, survival, cell adhesion, and mobility. BCAR3 functions synergistically with Cas to enhance Src activation and promote cell migration, and has been suggested to regulate Cas/Src association, Src kinase activity, and breast cancer adhesion signaling.
Background References:
  • van Agthoven T, van Agthoven TL, Dekker A, et al. Identification of BCAR3 by a random search for genes involved in antiestrogen resistnace of human breast cancer cells. EMBO J. 1998; 17:2799-808.
  • Riggins RB, Quilliam LA, and Bouton AH. Synergistic promotion of c-Src activation and cell migration by Cas and AND-34/BCAR3. J. Biol. Chem. 2003; 278:28264-73.
  • Schuh NR, Guerrero MS, Schrecengost RS, et al. BCAR3 regulates Src/p130Cas association, Src kinase activity, and breast cancer adhesion signaling. J. Biol. Chem. 2010; 285:2309-17.
Buffer:
BCAR3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
BCAR3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BCAR3.

The immunogen is located within amino acids 30 - 80 of BCAR3.
NCBI Gene ID #:
8412
NCBI Official Name:
breast cancer anti-estrogen resistance protein 3
NCBI Official Symbol:
BCAR3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
91 kDa
Protein Accession #:
NP_003558
Protein GI Number:
4502371
Purification:
BCAR3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.
Swissprot #:
O75815
User NOte:
Optimal dilutions for each application to be determined by the researcher.