ProSci

CBX4 Antibody

Product Code:
 
PSI-7007
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 2
Western blot analysis of CBX4 in human brain tissue lysate with CBX4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunofluorescence of CBX4 in human brain tissue with CBX4 antibody at 20 μg/mL.
❮❯

Western blot analysis of CBX4 in human brain tissue lysate with CBX4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of CBX4 in human brain tissue with CBX4 antibody at 20 μg/mL.

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CodeSizePrice
PSI-7007-0.02mg0.02mg£150.00
Quantity:
PSI-7007-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
CBX4 Antibody: PC2, NBP16, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Pc2
Application Note:
CBX4 antibody can be used for detection of CBX4 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CBX4 Antibody: Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.
Background References:
  • Simon JA and Tamkun JW. Programming off and on states in chromatin: mechanisms of Polycomb and trithorax group complexes. Curr. Opin. Genet. Dev. 2002;12:210-8.
  • Gieni RS and Hendzel MJ. Polycomb group protein gene silencing, non-coding RNA, stem cells, and cancer. Biochem. Cell Biol. 2009; 87:711-46.
  • Luis NM, Morey L, Mejetta S, et al. Regulation of human epidermal stem cell proliferation and senescence requires polycomb-dependent and -independent functions of Cbx4. Cell. Stem Cell 2011; 9:233-46.
  • Ismail IH, Gagn? JP, Caron MC, et al. CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA damage. Nucleic Acids Res. 2012 Mar 8.
Buffer:
CBX4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CBX4 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CBX4.

The immunogen is located within amino acids 50 - 100 of CBX4.
NCBI Gene ID #:
8535
NCBI Official Name:
chromobox homolog 4
NCBI Official Symbol:
CBX4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
NP_003646
Protein GI Number:
55770830
Purification:
CBX4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
Two alternatively spliced transcript variants have been observed.
Swissprot #:
O00257
User NOte:
Optimal dilutions for each application to be determined by the researcher.