ProSci

Pellino 1 Antibody

Product Code:
 
PSI-7071
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
1 / 3
Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 μg/ml.
3 / 3
Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 μg/ml.
❮❯

Western blot analysis of Pellino 1 in human liver tissue lysate with Pellino 1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of Pellino in HepG2 cells with Pellino 1 antibody at 2.5 μg/ml.
Immunofluorescence of Pellino 1 in HepG2 cells with Pellino 1 antibody at 20 μg/ml.

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CodeSizePrice
PSI-7071-0.02mg0.02mg£150.00
Quantity:
PSI-7071-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
Pellino 1 Antibody: PRISM, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-related intracellular-signaling molecule, Pellino-1
Application Note:
Pellino 1 antibody can be used for detection of Pellino 1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Pellino 1 Antibody: The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κB. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
Background References:
  • Moynagh PN. The Pellino family: IRAK E3 ligases with emerging roles in innate immune signaling. Trends Immunol. 2009; 30:33-42.
  • Jiang Z, Johnson J, Nie H, et al. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex. J. Biol. Chem. 2003; 278:10952-6
  • Butler MP, Hanly JA, and Moynagh PN. Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for Pellino proteins being ubiquitin-protein isopeptide ligases. J. Biol. Chem. 2007; 282:29729-37.
  • Goh ET, Arthur JS, Cheung PC, et al. Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem. J. 2012; 441:339-46
Buffer:
Pellino 1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal Pellino 1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Pellino 1.

The immunogen is located within amino acids 300 - 350 of Pellino 1.
NCBI Gene ID #:
57162
NCBI Official Name:
pellino E3 ubiquitin protein ligase 1
NCBI Official Symbol:
PELI1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
NP_065702
Protein GI Number:
11037063
Purification:
Pellino 1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q96FA3
User NOte:
Optimal dilutions for each application to be determined by the researcher.