ProSci

TRIM28 Antibody

Product Code:
 
PSI-2521
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 4
<strong>Figure 1 Immunofluorescence Validation of TRIM28 in HeLa Cells</strong><br>
2 / 4
<strong>Figure 2 WB Validation in Human Cell Lines </strong><br>
3 / 4
<strong>Figure 3 WB Validation in Mouse Tissue Lysate</strong><br>
4 / 4
<strong>Figure 4 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of rat tissue lysates. Antibodies: TRIM28 2521, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
❮❯

<strong>Figure 1 Immunofluorescence Validation of TRIM28 in HeLa Cells</strong><br>
<strong>Figure 2 WB Validation in Human Cell Lines </strong><br>
<strong>Figure 3 WB Validation in Mouse Tissue Lysate</strong><br>
<strong>Figure 4 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of rat tissue lysates. Antibodies: TRIM28 2521, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-2521-0.02mg0.02mg£150.00
Quantity:
PSI-2521-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Additional Names:
TRIM28 Antibody: KAP1, TF1B, RNF96, TIF1B, KAP1, Transcription intermediary factor 1-beta, E3 SUMO-protein ligase TRIM28, TIF1-beta
Application Note:
TRIM28 antibody can be used for detection of TRIM28 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
TRIM28 Antibody: TRIM28, also known as KAP-1, is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily and contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TRIM28 is a corepressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TRIM28 acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TRIM28 associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.
Background References:
  • Friedman JR, Fredericks WJ, Jensen DE, et al. KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 1996; 10:2067-78.
  • Cammas F, Mark M, Dolle P, et al. Mice lacking the transcriptional corepressor TIF1beta are defective in early postimplantation development. Dev. 2000; 127:2955-63.
  • Chang CJ, Chen YL, and Lee SC. Coactivator TIF1beta interacts with transcription factor C/EBPbeta and glucocorticoid receptor to induce alpha1-acid glycoprotein gene expression. Mol. Cell Biol. 1998; 18:5880-7
  • Groner AC, Meylan S, Ciuffi A, et al. KRAB-zinc finger proteins and KAP1 can mediate long-range transcriptional repression through heterochromatin spreading. PLoS Genet. 2010; 6:e1000869.
Buffer:
TRIM28 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal TRIM28 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TRIM28.

The immunogen is located within the last 50 amino acids of TRIM28.
NCBI Gene ID #:
10155
NCBI Official Name:
tripartite motif containing 28
NCBI Official Symbol:
TRIM28
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 92 kDa
Protein Accession #:
NP_005753
Protein GI Number:
5032179
Purification:
TRIM28 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least three isoforms of TRIM28 are known to exist; this antibody will detect all three isoforms
Swissprot #:
Q13263
User NOte:
Optimal dilutions for each application to be determined by the researcher.