ProSci

FOXP3 Antibody

Product Code:
 
PSI-7187
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
1 / 3
Western blot analysis of FOXP3 in A549 cell lysate with FOXP3 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of FOXP3 in human brain tissue with FOXP3 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of FOXP3 in human brain tissue with FOXP3 antibody at 20 μg/mL.<br><br>Green: FOXP3 Antibody (7187) <br> Blue: DAPI staining
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Western blot analysis of FOXP3 in A549 cell lysate with FOXP3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of FOXP3 in human brain tissue with FOXP3 antibody at 5 μg/mL.
Immunofluorescence of FOXP3 in human brain tissue with FOXP3 antibody at 20 μg/mL.<br><br>Green: FOXP3 Antibody (7187) <br> Blue: DAPI staining

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CodeSizePrice
PSI-7187-0.02mg0.02mg£150.00
Quantity:
PSI-7187-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
FOXP3 Antibody: JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2, Forkhead box protein P3, Scurfin
Application Note:
FOXP3 antibody can be used for detection of FOXP3 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
FOXP3 Antibody: FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Genetic mutations involving FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.
Background References:
  • Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 2001; 27:68-73
  • Schubert LA, Jeffery E, Zhang Y, et al. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J. Biol. Chem. 2001; 276:37672-9.
  • Gavin MA, Rasmussen JP, Fontenot JD, et al. Foxp3-dependent programme of regulatory T-cell differentiation. Nature 2007; 445:771-5
  • Kobayashi I, Shiari R, Yamada M, et al. Novel mutations of FOXP3 in two Japanese patients with immune dysregulation, polyendocrinopathy, enteropathy, X linked syndrome (IPEX). J. Med. Genet. 2001; 38:874-6.
Buffer:
FOXP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal FOXP3 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human FOXP3.

The immunogen is located within the last 50 amino acids of FOXP3.
NCBI Gene ID #:
50943
NCBI Official Name:
forkhead box P3
NCBI Official Symbol:
FOXP3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 47 kDa
Protein Accession #:
NP_054728
Protein GI Number:
31982943
Purification:
FOXP3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q9BZS1
User NOte:
Optimal dilutions for each application to be determined by the researcher.