ProSci

FOXA2 Antibody

Product Code:
 
PSI-7257
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
1 / 4
Western blot analysis of FOXA2 in human bladder tissue lysate with FOXA2 antibody at 1 μg/mL.
2 / 4
Immunofluorescence of FOXA2 in human bladder tissue with FOXA2 antibody at 20 μg/ml.
3 / 4
Immunohistochemistry of FOXA2 in mouse brain tissue with FOXA2 Antibody at 5 μg/mL.
4 / 4
Immunofluorescence of FOXA2 in mouse brain tissue with FOXA2 Antibody at 20 μg/mL. <br><br>Green: FOXA2 antibody (7257) <br> Red: Phylloidin staining<br> Blue: DAPI staining
❮❯

Western blot analysis of FOXA2 in human bladder tissue lysate with FOXA2 antibody at 1 μg/mL.
Immunofluorescence of FOXA2 in human bladder tissue with FOXA2 antibody at 20 μg/ml.
Immunohistochemistry of FOXA2 in mouse brain tissue with FOXA2 Antibody at 5 μg/mL.
Immunofluorescence of FOXA2 in mouse brain tissue with FOXA2 Antibody at 20 μg/mL. <br><br>Green: FOXA2 antibody (7257) <br> Red: Phylloidin staining<br> Blue: DAPI staining

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-7257-0.02mg0.02mg£150.00
Quantity:
PSI-7257-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Related Products
  • Show All

Further Information

Additional Names:
FOXA2 Antibody: HNF3B, TCF3B, HNF3B, Hepatocyte nuclear factor 3-beta, Forkhead box protein A2, HNF-3-beta
Application Note:
FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
FOXA2 Antibody: FOXA2 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. FOXA2 was initially identified through library screening as a closely related homolog of FOXA1. Both FOXA2 and FOXA1 act as transcriptional activators in adult liver and also play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation.
Background References:
  • Hannenhalli S and Kaestner KH. The evolution of Fox genes and their role in development and disease. Nat. Rev. Genet. 2009; 10:233-40.
  • Lai E, Prezioso VR, Tao WF, et al. Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head. Genes Dev. 1991; 5:416-27.
  • Sasaki H and Hogn BL. Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo. Dev. 1993; 118:47-59.
Buffer:
FOXA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (94%)
Immunogen:
FOXA2 antibody was raised against a 16 amino acid peptide near the center of human FOXA2 .

The immunogen is located within amino acids 280 - 330 of FOXA2.
NCBI Gene ID #:
3170
NCBI Official Name:
forkhead box A2
NCBI Official Symbol:
FOXA2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 51 kDa

Observed: 54 kDa
Protein Accession #:
NP_068556
Protein GI Number:
194394143
Purification:
FOXA2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family.
Swissprot #:
Q9Y261
User NOte:
Optimal dilutions for each application to be determined by the researcher.