ProSci

Neurochondrin Antibody

Product Code:
 
PSI-45-952
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-952 (0.3ug/ml) staining of HepG2 (A) and (1ug/ml) U251 cell lysate (B) + peptide (C) (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-952 (0.3ug/ml) staining of HepG2 (A) and (1ug/ml) U251 cell lysate (B) + peptide (C) (35ug protein in RIPA buffer). Detected by chemiluminescence.

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PSI-45-952-0.1mg0.1mg£626.00
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Further Information

Additional Names:
NCDN, neurochondrin , KIAA0607
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and U251 (calculated MW of 77.2kDa according to NP_001014841.1). An additional band of unknown identity was also observed in U251 lysates at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Kidney and Brain and NIH3T3 cell line for this particular batch.
Background References:
  • Dateki M, Horii T, Kasuya Y, Mochizuki R, Nagao Y, Ishida J, Sugiyama F, Tanimoto K, Yagami K, Imai H, Fukamizu A. Neurochondrin negatively regulates CaMKII phosphorylation, and nervous system-specific gene disruption results in epileptic seizure. J Biol Chem. 2005 May 27;280(21):20503-8. Epub 2005 Mar 24.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HYLLQVGSEKQKEP
NCBI Gene ID #:
23154
NCBI Official Name:
neurochondrin
NCBI Official Symbol:
NCDN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055099.1, NP_001014841.1
Protein GI Number:
24307949
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_055099.1; NP_001014841.1). Reported variants represent identical protein (NP_055099.1; NP_001014839.1).
Swissprot #:
Q9UBB6