ProSci

REV1L Antibody

Product Code:
 
PSI-46-281
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
46-281 (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
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46-281 (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

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PSI-46-281-0.1mg0.1mg£626.00
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Further Information

Additional Names:
REV1, REV1L, REV1-like (yeast), REV1, REV1 protein, REV1 (yeast homolog)- like, REV1 homolog (S. cerevisiae), FLJ21523, MGC163283, MGC26225, REV1- like
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6ug/ml.
Background References:
  • Masuda Y, Takahashi M, Tsunekuni N, Minami T, Sumii M, Miyagawa K, Kamiya K. Masuda Y, Takahashi M, Tsunekuni N, Minami T, Sumii M, Miyagawa K, Kamiya K. J Biol Chem. 2001 May 4;276(18):15051-8. Epub 2001 Jan 22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-VLQQTYGSTLKVT
NCBI Gene ID #:
51455
NCBI Official Name:
REV1, polymerase (DNA directed)
NCBI Official Symbol:
REV1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_057400.1, NP_001032961.1
Protein GI Number:
7706681
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms.
Swissprot #:
Q9UBZ9