ProSci

DBP5 Antibody

Product Code:
 
PSI-45-463
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-463 (0.5ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-463 (0.5ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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PSI-45-463-0.1mg0.1mg£626.00
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Further Information

Additional Names:
DDX19, DBP5, DDX19B, DEAD (Asp-Glu-Ala-As) box polypeptide 19B, RNAh , ATP-dependent RNA helicase DDX19, DEAD (Asp-Glu-Ala-As) box polypeptide 19, DEAD-box RNA helicase DEAD5, DEAD-box protein 5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog), yeast Dbp5 homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in lysates of human ovary and of cell line K562 (calculated MW of 50.5kDa according to NP_001014451.1). Recommended concentration: 0.5-1.5ug/ml.
Background References:
  • Cole CN, Scarcelli JJ. Transport of messenger RNA from the nucleus to the cytoplasm. Curr Opin Cell Biol. 2006 Jun;18(3):299-306. Epub 2006 May 8. Review.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DLPVDKDGNPDNETY
NCBI Gene ID #:
11269; 55308
NCBI Official Name:
DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B
NCBI Official Symbol:
DDX19B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_009173.1, NP_001014451.1, NP_001014449.1, NP_060802.1
Protein GI Number:
6005743
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise DDX19A (NP_060802.1) and all reported isoforms of DDX19B (NP_009173.1, NP_001014451.1 and NP_001014449.1).
Swissprot #:
Q9UMR2