ProSci

ICAM5 Precursor Antibody

Product Code:
 
PSI-45-758
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 1
45-758 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebral Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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45-758 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebral Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-45-758-0.1mg0.1mg£626.00
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Further Information

Additional Names:
ICAM5, intercellular adhesion molecule 5, telencephalin , TLCN, TLN , intercellular adhesion molecule 5, telencephalin
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2kDa according to NP_003250.2. The 200-250kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4ug/ml.
Background References:
  • Yoshihara Y, Oka S, Nemoto Y, Watanabe Y, Nagata S, Kagamiyama H, Mori K. An ICAM-related neuronal glycoprotein, telencephalin, with brain segment-specific expression. Neuron. 1994 Mar;12(3):541-53.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ESPAEGEVFAIQ
NCBI Gene ID #:
7087
NCBI Official Name:
intercellular adhesion molecule 5, telencephalin
NCBI Official Symbol:
ICAM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003250.3
Protein GI Number:
12545404
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9UMF0