Further Information
ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiquitin protein ligase ITCH
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot:Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A. Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination. Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
The immunogen for this antibody is: C-EIKSHDLKPNGGN
83737
itchy E3 ubiquitin protein ligase
ITCH
Homo sapiens
Liquid
NP_113671.3, NP_001244066.1, NP_001244067.1
27477109
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
.
Q96J02