Antibody Clonality:
Polyclonal
Applications:- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.
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![Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189). Western blot analysis of lysed extracts from HeLa cells untreated or treated with PMA using MKK3 (Phospho-Ser189).](https://www.prosci-inc.com/static-images/79-171.jpg)
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![<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Phospho-Ser189). <b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3 (Phospho-Ser189).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MKK3 (Phospho-Ser189).](https://www.prosci-inc.com/static-images/79-171a.jpg)
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This product comes from:
United States.
Typical lead time:
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Further Information
MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
- Wang W, et al. (2002) Mol Cell Biol ; 22 (10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3): 1247-55.
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
MKK3 (Phospho-Ser189) antibody was raised against a peptide sequence around phosphorylation site of serine 189 (V-D-S (p) -V-A) derived from Human MKK3.
Phospho-Specific
5606
mitogen-activated protein kinase kinase 3
MAP2K3
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
NP_002747.2
21618347
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Phospho-Specific
This antibody detects endogenous level of MKK3 only when phosphorylated at serine189.
P46734