Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 5
![46-777 (1ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. 46-777 (1ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](https://www.prosci-inc.com/static-images/46-777_1.jpg)
2 / 5
![](https://www.prosci-inc.com/static-images/46-777_2.jpg)
3 / 5
![](https://www.prosci-inc.com/static-images/46-777_3.jpg)
4 / 5
![46-777 (3.75ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. 46-777 (3.75ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](https://www.prosci-inc.com/static-images/46-777_4.jpg)
5 / 5
![46-777 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody. 46-777 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.](https://www.prosci-inc.com/static-images/46-777_5.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
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Further Information
DES, desmin, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55-60kDa band observed in Human, Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 53.5kDa according to Human NP_001918.3, Mouse NP_034173.1, Rat NP_071976.1 and 53.6kDa according to Pig NP_001001535.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5ug/ml. In paraffin embedded Human Heart, expression of Desmin is detected in myocardial muscle and not in adjacent vascular smooth muscle. Recommended concentration:3.75ug/ml. Data obtained from previous batch.
- Chen F, Chang R, Trivedi M, Capetanaki Y, Cryns VL. Caspase proteolysis of desmin produces a dominant-negative inhibitor of intermediate filaments and promotes apoptosis. J Biol Chem. 2003 Feb 28;278(9):6848-53.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Dog, Cow
The immunogen for this antibody is: C-RDGEVVSEATQQQHE
1674
desmin
DES
Homo sapiens
Liquid
NP_001918.3
55749932
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Signal Transduction ,Stem Cell
.
P17661